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KMID : 0982820050040010038
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Journal of Lung Cancer
2005 Volume.4 No. 1 p.38 ~ p.41
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Mutational Analysis of Caspase-7 and 8 Genes in Non-small Cell Lung Cancer
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Soung Young-Hwa
Lee Jong-Woo
Moon Seok-Whan
Jo Keon-Hyon
Wang Young-Pil
Kim Su-Young
Nam Suk-Woo
Park Won-Sang
Lee Jung-Young
Yoo Nam-Jin
Lee Sug-Hyung
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Abstract
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Purpose: Several lines of evidence have indicated that deregulation of apoptosis is involved in the mechanism of cancer development. Caspase-8 activation plays a central role in the initiation phase of apoptosis, while caspase-7 is one of the main execution phase caspases of apoptosis. The aim of this study was to explore the possibility that genetic alterations of the caspase-8 and caspase-7 genes are involved in the development of human non-small cell lung cancer (NSCLC).
Materials and Methods: We have analyzed the entire coding region of both the caspase-7 and caspase-8 genes to detect the somatic mutations in 100 NSCLCs by using polymerase chain reaction (PCR)- single strand conformation polymorphism (SSCP).
Results: The PCR-SSCP analysis detected no mutations in the entire coding regions of both the caspase-7 and caspase-8 genes in the NSCLCs.
Conclusion: The data presented here suggests that both the caspase-7 and caspase-8 genes may not be somatically mutated in human NSCLCs.
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KEYWORD
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Non-small cell lung cancer, Caspase-7, Caspase-8, Apoptosis, Mutation
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